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p75 magnetic beads  (Miltenyi Biotec)


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    Miltenyi Biotec p75 magnetic beads
    A) Schematic depicting the pipeline of the RNA sequencing experiment. Green arrows represent timepoints when organoids were sampled. Note that at day 8, neurocrestoids are dissociated into single cells, and NCCs are selected using p75NTR magnetic beads and added to the sample pool for transcriptomic analysis. B) Heatmap of differentially expressed genes (padj<0.05; |log2FC|>1; D0 vs <t>p75)</t> relating to pluripotent, NMP, NPB/Early NC, and NC populations over time. C) Heatmap of differentially expressed genes (padj<0.05; |log2FC|>1; D0 vs D8) relating to NT, NNE, CP, mesoderm, endoderm and NC populations in day 8 neurocrestoids. NNE: Non-neural ectoderm, CP: Cranial Placodes, Meso: Mesoderm, Endo: Endoderm, NC: Neural Crest. D) Heatmap of differentially expressed genes relating to Wnt canonical pathway ligands, Wnt non-canonical pathway ligands, Wnt canonical pathway effectors and regulators, FGF and BMP ligands over time. E) Bar chart depicting the change in expression (log2 fold change) in the NCC condition (p75 sorted) for all statistically significantly expressed Hox genes versus day 0. Different shades of green mark Hox genes relating to cranial, vagal and trunk axial positions. Hox genes are in descending order by log2 fold change in expression versus day 0. F) Example optical slices of 2 different organoids showcasing the ability of neurocrestoids in generating different subpopulations of NCCs at day 8. White arrowheads indicate SOX10-positive areas.
    P75 Magnetic Beads, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 94/100, based on 30 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/cd271+microbead+kit/CD271+MicroBead+Kit%2C+human/bio_rxiv__64898__2026__05__04__721958-303-9-17
    Average 94 stars, based on 30 article reviews
    p75 magnetic beads - by Bioz Stars, 2026-09
    94/100 stars

    Images

    1) Product Images from "Dynamic modelling of human neural crest development using a bioengineered stem cell organoid system"

    Article Title: Dynamic modelling of human neural crest development using a bioengineered stem cell organoid system

    Journal: bioRxiv

    doi: 10.64898/2026.05.04.721958

    A) Schematic depicting the pipeline of the RNA sequencing experiment. Green arrows represent timepoints when organoids were sampled. Note that at day 8, neurocrestoids are dissociated into single cells, and NCCs are selected using p75NTR magnetic beads and added to the sample pool for transcriptomic analysis. B) Heatmap of differentially expressed genes (padj<0.05; |log2FC|>1; D0 vs p75) relating to pluripotent, NMP, NPB/Early NC, and NC populations over time. C) Heatmap of differentially expressed genes (padj<0.05; |log2FC|>1; D0 vs D8) relating to NT, NNE, CP, mesoderm, endoderm and NC populations in day 8 neurocrestoids. NNE: Non-neural ectoderm, CP: Cranial Placodes, Meso: Mesoderm, Endo: Endoderm, NC: Neural Crest. D) Heatmap of differentially expressed genes relating to Wnt canonical pathway ligands, Wnt non-canonical pathway ligands, Wnt canonical pathway effectors and regulators, FGF and BMP ligands over time. E) Bar chart depicting the change in expression (log2 fold change) in the NCC condition (p75 sorted) for all statistically significantly expressed Hox genes versus day 0. Different shades of green mark Hox genes relating to cranial, vagal and trunk axial positions. Hox genes are in descending order by log2 fold change in expression versus day 0. F) Example optical slices of 2 different organoids showcasing the ability of neurocrestoids in generating different subpopulations of NCCs at day 8. White arrowheads indicate SOX10-positive areas.
    Figure Legend Snippet: A) Schematic depicting the pipeline of the RNA sequencing experiment. Green arrows represent timepoints when organoids were sampled. Note that at day 8, neurocrestoids are dissociated into single cells, and NCCs are selected using p75NTR magnetic beads and added to the sample pool for transcriptomic analysis. B) Heatmap of differentially expressed genes (padj<0.05; |log2FC|>1; D0 vs p75) relating to pluripotent, NMP, NPB/Early NC, and NC populations over time. C) Heatmap of differentially expressed genes (padj<0.05; |log2FC|>1; D0 vs D8) relating to NT, NNE, CP, mesoderm, endoderm and NC populations in day 8 neurocrestoids. NNE: Non-neural ectoderm, CP: Cranial Placodes, Meso: Mesoderm, Endo: Endoderm, NC: Neural Crest. D) Heatmap of differentially expressed genes relating to Wnt canonical pathway ligands, Wnt non-canonical pathway ligands, Wnt canonical pathway effectors and regulators, FGF and BMP ligands over time. E) Bar chart depicting the change in expression (log2 fold change) in the NCC condition (p75 sorted) for all statistically significantly expressed Hox genes versus day 0. Different shades of green mark Hox genes relating to cranial, vagal and trunk axial positions. Hox genes are in descending order by log2 fold change in expression versus day 0. F) Example optical slices of 2 different organoids showcasing the ability of neurocrestoids in generating different subpopulations of NCCs at day 8. White arrowheads indicate SOX10-positive areas.

    Techniques Used: RNA Sequencing, Magnetic Beads, Expressing

    Related Articles

    Magnetic Cell Separation:

    Article Title: Two different forms of inherited human TCRα chain deficiency
    Article Snippet: .. Enrichment in transduced cells was achieved with a MACS Column and the CD271 MicroBead Kit (no. 130-099-023; Miltenyi Biotec), according to the manufacturer’s protocol. ..

    Article Title: Supporting Information for Engineering an inducible leukemia-associated fusion protein enables large-scale ex vivo production of functional human phagocytes
    Article Snippet: .. Thereafter, the collected cells were enriched using the CD271 MicroBead Kit and magnetic enrichment by the MACS-system (both Miltenyi Biotec, Bergisch Gladbach, Germany) following the manufacturer’s protocol. .. The immunophenotype of the hMSC was confirmed using CD271 (130-113-418, Miltenyi Biotec, Bergisch Gladbach, Germany). hMSC were cultured and expanded in StemMACS MSC Expansion media (Miltenyi Biotec, Bergisch Gladbach, Germany).

    Article Title: Two different forms of inherited human TCRα chain deficiency
    Article Snippet: Transduction efficiency was assessed by flow cytometry with an anti-CD271 antibody (no. 557196; 1:500; BD). .. Enrichment in transduced cells was achieved with a MACS Column and the CD271 MicroBead Kit (no. 130-099-023; Miltenyi Biotec), according to the manufacturer’s protocol. ..

    Article Title: Differential inflammatory conditioning of the bone marrow by acute myeloid leukemia and its impact on progression
    Article Snippet: .. Afterwards, collected cells were enriched using the CD271 MicroBead Kit (Miltenyi Biotec, Bergisch Gladbach), following the manufacturer’s protocol, and separated by magnetic enrichment using the magnet activated cell sorting (MACS) system (Miltenyi Biotec, Bergisch Gladbach). .. The CD271+ cells were cultured and expanded in StemMACS MSC Expansion medium (Miltenyi Biotec, Bergisch Gladbach).

    Isolation:

    Article Title: “Cellularity as a predictive tool for mesenchymal stem cell concentration in bone marrow concentrates: Implications for regenerative medicine”
    Article Snippet: .. In the next step, CD271+ cells were isolated from the remaining cell pellet using the CD271 MicroBead Kit (Miltenyi Biotec, Bergisch Gladbach, Germany) according to the manufacturer's protocol. ..

    Article Title: “Cellularity as a predictive tool for mesenchymal stem cell concentration in bone marrow concentrates: Implications for regenerative medicine”
    Article Snippet: .. In the next step, CD271 + cells were isolated from the remaining cell pellet using the CD271 MicroBead Kit (Miltenyi Biotec, Bergisch Gladbach, Germany) according to the manufacturer's protocol. ..

    FACS:

    Article Title: Differential inflammatory conditioning of the bone marrow by acute myeloid leukemia and its impact on progression
    Article Snippet: .. Afterwards, collected cells were enriched using the CD271 MicroBead Kit (Miltenyi Biotec, Bergisch Gladbach), following the manufacturer’s protocol, and separated by magnetic enrichment using the magnet activated cell sorting (MACS) system (Miltenyi Biotec, Bergisch Gladbach). .. The CD271+ cells were cultured and expanded in StemMACS MSC Expansion medium (Miltenyi Biotec, Bergisch Gladbach).



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    A) Schematic depicting the pipeline of the RNA sequencing experiment. Green arrows represent timepoints when organoids were sampled. Note that at day 8, neurocrestoids are dissociated into single cells, and NCCs are selected using p75NTR magnetic beads and added to the sample pool for transcriptomic analysis. B) Heatmap of differentially expressed genes (padj<0.05; |log2FC|>1; D0 vs <t>p75)</t> relating to pluripotent, NMP, NPB/Early NC, and NC populations over time. C) Heatmap of differentially expressed genes (padj<0.05; |log2FC|>1; D0 vs D8) relating to NT, NNE, CP, mesoderm, endoderm and NC populations in day 8 neurocrestoids. NNE: Non-neural ectoderm, CP: Cranial Placodes, Meso: Mesoderm, Endo: Endoderm, NC: Neural Crest. D) Heatmap of differentially expressed genes relating to Wnt canonical pathway ligands, Wnt non-canonical pathway ligands, Wnt canonical pathway effectors and regulators, FGF and BMP ligands over time. E) Bar chart depicting the change in expression (log2 fold change) in the NCC condition (p75 sorted) for all statistically significantly expressed Hox genes versus day 0. Different shades of green mark Hox genes relating to cranial, vagal and trunk axial positions. Hox genes are in descending order by log2 fold change in expression versus day 0. F) Example optical slices of 2 different organoids showcasing the ability of neurocrestoids in generating different subpopulations of NCCs at day 8. White arrowheads indicate SOX10-positive areas.
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    Image Search Results


    A) Schematic depicting the pipeline of the RNA sequencing experiment. Green arrows represent timepoints when organoids were sampled. Note that at day 8, neurocrestoids are dissociated into single cells, and NCCs are selected using p75NTR magnetic beads and added to the sample pool for transcriptomic analysis. B) Heatmap of differentially expressed genes (padj<0.05; |log2FC|>1; D0 vs p75) relating to pluripotent, NMP, NPB/Early NC, and NC populations over time. C) Heatmap of differentially expressed genes (padj<0.05; |log2FC|>1; D0 vs D8) relating to NT, NNE, CP, mesoderm, endoderm and NC populations in day 8 neurocrestoids. NNE: Non-neural ectoderm, CP: Cranial Placodes, Meso: Mesoderm, Endo: Endoderm, NC: Neural Crest. D) Heatmap of differentially expressed genes relating to Wnt canonical pathway ligands, Wnt non-canonical pathway ligands, Wnt canonical pathway effectors and regulators, FGF and BMP ligands over time. E) Bar chart depicting the change in expression (log2 fold change) in the NCC condition (p75 sorted) for all statistically significantly expressed Hox genes versus day 0. Different shades of green mark Hox genes relating to cranial, vagal and trunk axial positions. Hox genes are in descending order by log2 fold change in expression versus day 0. F) Example optical slices of 2 different organoids showcasing the ability of neurocrestoids in generating different subpopulations of NCCs at day 8. White arrowheads indicate SOX10-positive areas.

    Journal: bioRxiv

    Article Title: Dynamic modelling of human neural crest development using a bioengineered stem cell organoid system

    doi: 10.64898/2026.05.04.721958

    Figure Lengend Snippet: A) Schematic depicting the pipeline of the RNA sequencing experiment. Green arrows represent timepoints when organoids were sampled. Note that at day 8, neurocrestoids are dissociated into single cells, and NCCs are selected using p75NTR magnetic beads and added to the sample pool for transcriptomic analysis. B) Heatmap of differentially expressed genes (padj<0.05; |log2FC|>1; D0 vs p75) relating to pluripotent, NMP, NPB/Early NC, and NC populations over time. C) Heatmap of differentially expressed genes (padj<0.05; |log2FC|>1; D0 vs D8) relating to NT, NNE, CP, mesoderm, endoderm and NC populations in day 8 neurocrestoids. NNE: Non-neural ectoderm, CP: Cranial Placodes, Meso: Mesoderm, Endo: Endoderm, NC: Neural Crest. D) Heatmap of differentially expressed genes relating to Wnt canonical pathway ligands, Wnt non-canonical pathway ligands, Wnt canonical pathway effectors and regulators, FGF and BMP ligands over time. E) Bar chart depicting the change in expression (log2 fold change) in the NCC condition (p75 sorted) for all statistically significantly expressed Hox genes versus day 0. Different shades of green mark Hox genes relating to cranial, vagal and trunk axial positions. Hox genes are in descending order by log2 fold change in expression versus day 0. F) Example optical slices of 2 different organoids showcasing the ability of neurocrestoids in generating different subpopulations of NCCs at day 8. White arrowheads indicate SOX10-positive areas.

    Article Snippet: Additionally, NCCs were sorted from day 8 neurocrestoids using p75 magnetic beads as per the manufacturer’s instructions (Miltenyi Biotec, 130-099-023).

    Techniques: RNA Sequencing, Magnetic Beads, Expressing

    A) Schematic overview of the pipeline for generating neurocrestoids using custom-fabricated PDMS microwells. Prior to the start of the differentiation protocol, PDMS microwells are fabricated from custom-made resin moulds to aggregate hPSCs into spheroids as previously described . B) Overview of the CAD design for 400x400x250µm inverted pyramidal-shaped PDMS microwells. C) Images of the custom-made resin mould and resulting PDMS microwells. D) Graph indicating the sphere diameter (median 243µm), accompanied by an image of spheroids in PDMS microwells at 24 hours after seeding. Points represent single organoids (n=105). E) Schematic depicting the protocol to generate hPSC-derived neuroepithelial organoids, termed neurocrestoids, capable of producing NCCs in 3D. F) Representative brightfield images during the first 7 days of the differentiation process. G) 3D rendering from a whole-mount immunostained suspension organoid presenting SOX10-positive regions, demonstrating its ability to generate NCCs in 3D. H) A representative optical slice from the organoid in F) showing internal epithelial structures from which SOX10-positive cells emanate (yellow arrowhead). Scale bar = 100µm.

    Journal: bioRxiv

    Article Title: Dynamic modelling of human neural crest development using a bioengineered stem cell organoid system

    doi: 10.64898/2026.05.04.721958

    Figure Lengend Snippet: A) Schematic overview of the pipeline for generating neurocrestoids using custom-fabricated PDMS microwells. Prior to the start of the differentiation protocol, PDMS microwells are fabricated from custom-made resin moulds to aggregate hPSCs into spheroids as previously described . B) Overview of the CAD design for 400x400x250µm inverted pyramidal-shaped PDMS microwells. C) Images of the custom-made resin mould and resulting PDMS microwells. D) Graph indicating the sphere diameter (median 243µm), accompanied by an image of spheroids in PDMS microwells at 24 hours after seeding. Points represent single organoids (n=105). E) Schematic depicting the protocol to generate hPSC-derived neuroepithelial organoids, termed neurocrestoids, capable of producing NCCs in 3D. F) Representative brightfield images during the first 7 days of the differentiation process. G) 3D rendering from a whole-mount immunostained suspension organoid presenting SOX10-positive regions, demonstrating its ability to generate NCCs in 3D. H) A representative optical slice from the organoid in F) showing internal epithelial structures from which SOX10-positive cells emanate (yellow arrowhead). Scale bar = 100µm.

    Article Snippet: Additionally, NCCs were sorted from day 8 neurocrestoids using p75 magnetic beads as per the manufacturer’s instructions (Miltenyi Biotec, 130-099-023).

    Techniques: Derivative Assay, Suspension

    A) Schematic depicting the pipeline of the RNA sequencing experiment. Green arrows represent timepoints when organoids were sampled. Note that at day 8, neurocrestoids are dissociated into single cells, and NCCs are selected using p75NTR magnetic beads and added to the sample pool for transcriptomic analysis. B) Heatmap of differentially expressed genes (padj<0.05; |log2FC|>1; D0 vs p75) relating to pluripotent, NMP, NPB/Early NC, and NC populations over time. C) Heatmap of differentially expressed genes (padj<0.05; |log2FC|>1; D0 vs D8) relating to NT, NNE, CP, mesoderm, endoderm and NC populations in day 8 neurocrestoids. NNE: Non-neural ectoderm, CP: Cranial Placodes, Meso: Mesoderm, Endo: Endoderm, NC: Neural Crest. D) Heatmap of differentially expressed genes relating to Wnt canonical pathway ligands, Wnt non-canonical pathway ligands, Wnt canonical pathway effectors and regulators, FGF and BMP ligands over time. E) Bar chart depicting the change in expression (log2 fold change) in the NCC condition (p75 sorted) for all statistically significantly expressed Hox genes versus day 0. Different shades of green mark Hox genes relating to cranial, vagal and trunk axial positions. Hox genes are in descending order by log2 fold change in expression versus day 0. F) Example optical slices of 2 different organoids showcasing the ability of neurocrestoids in generating different subpopulations of NCCs at day 8. White arrowheads indicate SOX10-positive areas.

    Journal: bioRxiv

    Article Title: Dynamic modelling of human neural crest development using a bioengineered stem cell organoid system

    doi: 10.64898/2026.05.04.721958

    Figure Lengend Snippet: A) Schematic depicting the pipeline of the RNA sequencing experiment. Green arrows represent timepoints when organoids were sampled. Note that at day 8, neurocrestoids are dissociated into single cells, and NCCs are selected using p75NTR magnetic beads and added to the sample pool for transcriptomic analysis. B) Heatmap of differentially expressed genes (padj<0.05; |log2FC|>1; D0 vs p75) relating to pluripotent, NMP, NPB/Early NC, and NC populations over time. C) Heatmap of differentially expressed genes (padj<0.05; |log2FC|>1; D0 vs D8) relating to NT, NNE, CP, mesoderm, endoderm and NC populations in day 8 neurocrestoids. NNE: Non-neural ectoderm, CP: Cranial Placodes, Meso: Mesoderm, Endo: Endoderm, NC: Neural Crest. D) Heatmap of differentially expressed genes relating to Wnt canonical pathway ligands, Wnt non-canonical pathway ligands, Wnt canonical pathway effectors and regulators, FGF and BMP ligands over time. E) Bar chart depicting the change in expression (log2 fold change) in the NCC condition (p75 sorted) for all statistically significantly expressed Hox genes versus day 0. Different shades of green mark Hox genes relating to cranial, vagal and trunk axial positions. Hox genes are in descending order by log2 fold change in expression versus day 0. F) Example optical slices of 2 different organoids showcasing the ability of neurocrestoids in generating different subpopulations of NCCs at day 8. White arrowheads indicate SOX10-positive areas.

    Article Snippet: Additionally, NCCs were sorted from day 8 neurocrestoids using p75 magnetic beads as per the manufacturer’s instructions (Miltenyi Biotec, 130-099-023).

    Techniques: RNA Sequencing, Magnetic Beads, Expressing

    A) Heatmap of differentially expressed EMT genes (D8 vs D0) through the neurocrestoid differentiation process, including Cadherin isoforms. B) Representative Z-stacks of day 8 neurocrestoids from 3 independent neurocrestoid experimental replicates, along with zoomed-in images of the areas indicated by the white dashed squares. Note the presence of SOX9-positive cells in epithelial structures immediately adjacent to SOX10 populations and separated by a double-positive cell layer (red asterisks). C) Representative images of neurocrestoids through days 7 to 10 of the differentiation protocol, immunolabelled for SOX9, SOX10 and nuclei (DAPI). SOX9 populations appear at day 8 (white arrowhead). Accompanying is a zoomed-in image of a neurocrestoid at day 10 (i), which presents SOX9-positive cells in the edges, a double-positive layer of cells (red arrowhead) and SOX10 populations towards the organoid core. D) Representative maximum intensity projection of an HCR on an E9.5 Wnt1::cre +/tg ; Rosa26R mTmG mouse embryo labelling GFP (NCCs), Sox9, Sox10 and nuclei (Hoechst) in the trunk regions (somites 1 to 10).

    Journal: bioRxiv

    Article Title: Dynamic modelling of human neural crest development using a bioengineered stem cell organoid system

    doi: 10.64898/2026.05.04.721958

    Figure Lengend Snippet: A) Heatmap of differentially expressed EMT genes (D8 vs D0) through the neurocrestoid differentiation process, including Cadherin isoforms. B) Representative Z-stacks of day 8 neurocrestoids from 3 independent neurocrestoid experimental replicates, along with zoomed-in images of the areas indicated by the white dashed squares. Note the presence of SOX9-positive cells in epithelial structures immediately adjacent to SOX10 populations and separated by a double-positive cell layer (red asterisks). C) Representative images of neurocrestoids through days 7 to 10 of the differentiation protocol, immunolabelled for SOX9, SOX10 and nuclei (DAPI). SOX9 populations appear at day 8 (white arrowhead). Accompanying is a zoomed-in image of a neurocrestoid at day 10 (i), which presents SOX9-positive cells in the edges, a double-positive layer of cells (red arrowhead) and SOX10 populations towards the organoid core. D) Representative maximum intensity projection of an HCR on an E9.5 Wnt1::cre +/tg ; Rosa26R mTmG mouse embryo labelling GFP (NCCs), Sox9, Sox10 and nuclei (Hoechst) in the trunk regions (somites 1 to 10).

    Article Snippet: Additionally, NCCs were sorted from day 8 neurocrestoids using p75 magnetic beads as per the manufacturer’s instructions (Miltenyi Biotec, 130-099-023).

    Techniques:

    A) Time-lapse images of a live plated neurocrestoid with nuclei stained with SPY650-DNA. B) Representative fluorescence images at day 14 of plated neurocrestoids immunostained for SOX10 and nuclei (DAPI). C) Bar chart depicting percentage of SOX10 positive cells amongst the total nuclei (DAPI) in plated neurocrestoids at day 14 across experiments from 3 independent hPSC lines (mean ± S.D). Each point represents the average across n=5-18 organoids per individual experiment. F) Neurocrestoids plated at day 14 and stained against HOXC9, SOX10 and nuclei (DAPI). E) Representative immunofluorescence images of mouse trunk explant and human neurocrestoid-derived NCCs stained for SOX10. F) Bar charts indicating the area, circularity and solidity values for neurocrestoid-derived human and mouse trunk NC nuclei (mean ± S.D). Points represent the average value per field of view. For the human datasets, a total of 38 datapoints are plotted representing the average value per field of view, 5 images per sample, 3-5 organoids per cell line from 3 hiPSC lines (Control 3, KOLFC21 and KOLF2.1J) in 3 experimental blocks. The mouse dataset consists of the average of 10 cells per image, 10 images per explant in 3 mouse trunk NC explants cultured for 48 to 72 hours in 2 experimental blocks. Non-parametric Mann-Whitney test to test for significance between conditions, **P<0.01, ns: non-significant.

    Journal: bioRxiv

    Article Title: Dynamic modelling of human neural crest development using a bioengineered stem cell organoid system

    doi: 10.64898/2026.05.04.721958

    Figure Lengend Snippet: A) Time-lapse images of a live plated neurocrestoid with nuclei stained with SPY650-DNA. B) Representative fluorescence images at day 14 of plated neurocrestoids immunostained for SOX10 and nuclei (DAPI). C) Bar chart depicting percentage of SOX10 positive cells amongst the total nuclei (DAPI) in plated neurocrestoids at day 14 across experiments from 3 independent hPSC lines (mean ± S.D). Each point represents the average across n=5-18 organoids per individual experiment. F) Neurocrestoids plated at day 14 and stained against HOXC9, SOX10 and nuclei (DAPI). E) Representative immunofluorescence images of mouse trunk explant and human neurocrestoid-derived NCCs stained for SOX10. F) Bar charts indicating the area, circularity and solidity values for neurocrestoid-derived human and mouse trunk NC nuclei (mean ± S.D). Points represent the average value per field of view. For the human datasets, a total of 38 datapoints are plotted representing the average value per field of view, 5 images per sample, 3-5 organoids per cell line from 3 hiPSC lines (Control 3, KOLFC21 and KOLF2.1J) in 3 experimental blocks. The mouse dataset consists of the average of 10 cells per image, 10 images per explant in 3 mouse trunk NC explants cultured for 48 to 72 hours in 2 experimental blocks. Non-parametric Mann-Whitney test to test for significance between conditions, **P<0.01, ns: non-significant.

    Article Snippet: Additionally, NCCs were sorted from day 8 neurocrestoids using p75 magnetic beads as per the manufacturer’s instructions (Miltenyi Biotec, 130-099-023).

    Techniques: Staining, Fluorescence, Immunofluorescence, Derivative Assay, Control, Cell Culture, MANN-WHITNEY

    A) Integration of neurocrestoids with PDMS micropatterned substrates allow for selective guidance and live visualisation of NC migratory populations. After 8 days in a rotary culture, differentiating neurocrestoids are plated in Matrigel-coated PDMS microgrooves for an additional 6 days of culturing. B) Violin plot showing the levels of eccentricity of SOX10-positive nuclei across different PDMS topographies. Datapoints represent the average eccentricity of all SOX10-positive nuclei within each field of view (n=5-250) in 15-17 technical replicates from 1 hiPSC line (Control 3) in 1 experimental block. Ordinary one-way ANOVA with Tukey’s multiple comparisons to test for significance between conditions. C) Representative image of the edge of a day 14 neurocrestoid plated on 10x10µm grooves. D) Violin plot depicting the degree of orientation of SOX10-positive nuclei of plated neurocrestoids in different PDMS topographies, where microgrooves have been set at 90°). Each violin plot represents a set of nuclei (n=5-250) in 15-17 technical replicates from 1 hiPSC line (Control 3) in 1 experimental block. Ordinary one-way ANOVA with Tukey’s multiple comparisons to test for significance between conditions. *P<0.5, **P<0.01, ***P<0.001, ****P<0.0001, ns= non-significant. E) Representative images of day 14 NCCs from plated neurocrestoids in 3x10, 10x10, 25x10 and 50x10µm PDMS grooves and flat PDMS and tissue culture plastics as controls. Samples are stained for SOX10 and nuclei (DAPI). F) Representative images of the area adjacent to the organoid core in neurocrestoids plated in 10x10 and 50x10μm PDMS microgrooves. Note that wider grooves can host SOX10-negative cells, resulting in restriction of SOX10-positive cell migration (red arrowheads). In narrower grooves, SOX10-negative cells do not fit, allowing for NCCs to migrate underneath along the grooves (green arrowheads). G) Representative fluorescence images after 48 hours of mouse trunk NC explants plated on 10x10µm grooves, accompanied by zoomed-in images of the areas delimited with a yellow dashed square. H) Schematic overview of the pipeline to plate neurocrestoids onto a PDMS multigroove star device. Organoids are positioned in the flat PDMS centre of the device using custom-made PDMS plating devices, which are removed once the organoids attach to the substrate. I) Representative images of a neurocrestoid plated in a multigroove PDMS device. The organoid is stained for SOX10, Actin (Phalloidin555) and nuclei (DAPI).

    Journal: bioRxiv

    Article Title: Dynamic modelling of human neural crest development using a bioengineered stem cell organoid system

    doi: 10.64898/2026.05.04.721958

    Figure Lengend Snippet: A) Integration of neurocrestoids with PDMS micropatterned substrates allow for selective guidance and live visualisation of NC migratory populations. After 8 days in a rotary culture, differentiating neurocrestoids are plated in Matrigel-coated PDMS microgrooves for an additional 6 days of culturing. B) Violin plot showing the levels of eccentricity of SOX10-positive nuclei across different PDMS topographies. Datapoints represent the average eccentricity of all SOX10-positive nuclei within each field of view (n=5-250) in 15-17 technical replicates from 1 hiPSC line (Control 3) in 1 experimental block. Ordinary one-way ANOVA with Tukey’s multiple comparisons to test for significance between conditions. C) Representative image of the edge of a day 14 neurocrestoid plated on 10x10µm grooves. D) Violin plot depicting the degree of orientation of SOX10-positive nuclei of plated neurocrestoids in different PDMS topographies, where microgrooves have been set at 90°). Each violin plot represents a set of nuclei (n=5-250) in 15-17 technical replicates from 1 hiPSC line (Control 3) in 1 experimental block. Ordinary one-way ANOVA with Tukey’s multiple comparisons to test for significance between conditions. *P<0.5, **P<0.01, ***P<0.001, ****P<0.0001, ns= non-significant. E) Representative images of day 14 NCCs from plated neurocrestoids in 3x10, 10x10, 25x10 and 50x10µm PDMS grooves and flat PDMS and tissue culture plastics as controls. Samples are stained for SOX10 and nuclei (DAPI). F) Representative images of the area adjacent to the organoid core in neurocrestoids plated in 10x10 and 50x10μm PDMS microgrooves. Note that wider grooves can host SOX10-negative cells, resulting in restriction of SOX10-positive cell migration (red arrowheads). In narrower grooves, SOX10-negative cells do not fit, allowing for NCCs to migrate underneath along the grooves (green arrowheads). G) Representative fluorescence images after 48 hours of mouse trunk NC explants plated on 10x10µm grooves, accompanied by zoomed-in images of the areas delimited with a yellow dashed square. H) Schematic overview of the pipeline to plate neurocrestoids onto a PDMS multigroove star device. Organoids are positioned in the flat PDMS centre of the device using custom-made PDMS plating devices, which are removed once the organoids attach to the substrate. I) Representative images of a neurocrestoid plated in a multigroove PDMS device. The organoid is stained for SOX10, Actin (Phalloidin555) and nuclei (DAPI).

    Article Snippet: Additionally, NCCs were sorted from day 8 neurocrestoids using p75 magnetic beads as per the manufacturer’s instructions (Miltenyi Biotec, 130-099-023).

    Techniques: Control, Blocking Assay, Staining, Migration, Fluorescence

    Immunofluorescence characterisation of oesophageal CSCs indicating the expression of CD44, CD90, and CD271 oesophageal CSC surface markers. The micrographs represent three independent assays (50 μm scale bar and magnification: 200x).

    Journal: Frontiers in Immunology

    Article Title: Cell death mechanisms induced by gold nano-immunoconjugates-mediated photodynamic therapy against human oesophageal cancer stem cells

    doi: 10.3389/fimmu.2025.1585251

    Figure Lengend Snippet: Immunofluorescence characterisation of oesophageal CSCs indicating the expression of CD44, CD90, and CD271 oesophageal CSC surface markers. The micrographs represent three independent assays (50 μm scale bar and magnification: 200x).

    Article Snippet: The CSCs were isolated from the HKESC-1 cells utilising the CD44, CD90, and CD271 Microbeads Kits (130-095-194/130-096-253/130-099-023, Miltenyi Biotec) via a magnetic separation method.

    Techniques: Immunofluorescence, Expressing

    Nano-immuno-conjugate characterization. TEM analysis of AuNPs (A) and NIC (B) . (C) Spectra analysis of AuNPs, Anti-CD271 antibody, AlPcS 4 Cl, and NIC showing Anti-CD271 antibody at 278nm, a red shift of AuNP from 520 nm to 542 nm in the NIC and AlPcS 4 Cl at 350 nm and 672 nm.

    Journal: Frontiers in Immunology

    Article Title: Cell death mechanisms induced by gold nano-immunoconjugates-mediated photodynamic therapy against human oesophageal cancer stem cells

    doi: 10.3389/fimmu.2025.1585251

    Figure Lengend Snippet: Nano-immuno-conjugate characterization. TEM analysis of AuNPs (A) and NIC (B) . (C) Spectra analysis of AuNPs, Anti-CD271 antibody, AlPcS 4 Cl, and NIC showing Anti-CD271 antibody at 278nm, a red shift of AuNP from 520 nm to 542 nm in the NIC and AlPcS 4 Cl at 350 nm and 672 nm.

    Article Snippet: The CSCs were isolated from the HKESC-1 cells utilising the CD44, CD90, and CD271 Microbeads Kits (130-095-194/130-096-253/130-099-023, Miltenyi Biotec) via a magnetic separation method.

    Techniques: